免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Immunoglobulin G (IgG)
- 编号CEA544Gu
- 物种Cavia (Guinea pig ,豚鼠) 相同的名称,不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围1.23-100ug/mL
- 灵敏度最小可检测剂量小于等于0.48ug/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 1840 ¥ 2628 ¥ 11826 ¥ 22338 ¥ 183960
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
本试剂盒用于检测免疫球蛋白G(IgG),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的免疫球蛋白G(IgG)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 86-101 | 95 |
EDTA plasma(n=5) | 90-102 | 97 |
heparin plasma(n=5) | 84-91 | 87 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的免疫球蛋白G(IgG),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中免疫球蛋白G(IgG)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 79-96% | 80-98% | 86-105% | 89-101% |
EDTA plasma(n=5) | 86-99% | 95-102% | 86-95% | 93-101% |
heparin plasma(n=5) | 84-101% | 92-101% | 78-96% | 87-104% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板5次;
4. 加TMB底物90µL,37°C孵育10-20分钟;
5. 加终止液50µL,立即450nm读数。
实验原理
将抗原包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的免疫球蛋白G(IgG)与连接于固相载体上的抗原结合,然后加入HRP标记的二抗,彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的免疫球蛋白G(IgG)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
增值服务
相关产品
编号 | 适用物种:Cavia (Guinea pig ,豚鼠) | 应用(仅供研究使用,不用于临床诊断!) |
NPA544Gu01 | 免疫球蛋白G(IgG)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA544Gu01 | 免疫球蛋白G(IgG)多克隆抗体 | WB; IHC; ICC; IP. |
SAA544Gu09 | 兔抗豚鼠IgG多克隆抗体(辣根过氧化物酶标记) | WB; IHC; ICC. |
SAA544Gu07 | 兔抗豚鼠IgG多克隆抗体(生物素标记) | WB; IHC; ICC. |
SAA544Gu08 | 兔抗豚鼠IgG多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAA544Gu21 | 免疫球蛋白G(IgG)单克隆抗体 | WB; IHC; ICC; IP. |
CEA544Gu | 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA544Gu | 免疫球蛋白G(IgG)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
KSA544Gu11 | 免疫球蛋白G(IgG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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