免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Immunoglobulin G (IgG)
- 编号CEA544Gu
- 物种Cavia (Guinea pig ，豚鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围1.23-100ug/mL
- 灵敏度最小可检测剂量小于等于0.48ug/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 1840 ¥ 2628 ¥ 11826 ¥ 22338 ¥ 183960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测免疫球蛋白G(IgG)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的免疫球蛋白G(IgG)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 95 |
| EDTA plasma(n=5) | 90-102 | 97 |
| heparin plasma(n=5) | 84-91 | 87 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的免疫球蛋白G(IgG)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中免疫球蛋白G(IgG)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-96% | 80-98% | 86-105% | 89-101% |
| EDTA plasma(n=5) | 86-99% | 95-102% | 86-95% | 93-101% |
| heparin plasma(n=5) | 84-101% | 92-101% | 78-96% | 87-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板5次；
4. 加TMB底物90µL，37°C孵育10-20分钟；
5. 加终止液50µL，立即450nm读数。
实验原理
将抗原包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的免疫球蛋白G(IgG)与连接于固相载体上的抗原结合，然后加入HRP标记的二抗，彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的免疫球蛋白G(IgG)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Cavia (Guinea pig ，豚鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| NPA544Gu01 | 免疫球蛋白G(IgG)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA544Gu01 | 免疫球蛋白G(IgG)多克隆抗体 | WB; IHC; ICC; IP. |
| SAA544Gu09 | 兔抗豚鼠IgG多克隆抗体(辣根过氧化物酶标记) | WB; IHC; ICC. |
| SAA544Gu07 | 兔抗豚鼠IgG多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SAA544Gu08 | 兔抗豚鼠IgG多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAA544Gu21 | 免疫球蛋白G(IgG)单克隆抗体 | WB; IHC; ICC; IP. |
| CEA544Gu | 免疫球蛋白G(IgG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA544Gu | 免疫球蛋白G(IgG)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| KSA544Gu11 | 免疫球蛋白G(IgG)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
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