细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)

CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor

  • 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA548Ca.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测细胞间粘附分子1(ICAM1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的细胞间粘附分子1(ICAM1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 89-98 93
EDTA plasma(n=5) 78-102 81
heparin plasma(n=5) 79-103 91

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的细胞间粘附分子1(ICAM1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中细胞间粘附分子1(ICAM1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 84-103% 88-102% 81-99% 89-103%
EDTA plasma(n=5) 91-101% 90-102% 78-96% 98-105%
heparin plasma(n=5) 86-93% 80-102% 89-97% 85-93%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将细胞间粘附分子1(ICAM1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的细胞间粘附分子1(ICAM1)与连接于固相载体上的抗体结合,然后加入生物素化的细胞间粘附分子1(ICAM1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的细胞间粘附分子1(ICAM1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Canis familiaris; Canine (Dog,犬) 应用(仅供研究使用,不用于临床诊断!)
RPA548Ca01 细胞间粘附分子1(ICAM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA548Ca01 细胞间粘附分子1(ICAM1)多克隆抗体 WB; IHC; ICC; IP.
MAA548Ca21 细胞间粘附分子1(ICAM1)单克隆抗体 WB; IHC; ICC; IP.
SEA548Ca 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
PGA548Ca01 细胞间粘附分子1(ICAM1)引物对 PCR

参考文献

杂志 参考文献
Amran et al. Lipids in Health and Disease Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. [BioMed: 1476511X]
Evidence-based Complementary and Alternative Medicine Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats [PubMed: PMC3703719]
British Journal of Medical and Health Sciences EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS [Researchgate: Source]
Cytokine Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression. [Pubmed: 23434274]
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192]
Journal of Veterinary Internal Medicine Markers of coagulation activation, endothelial stimulation, and inflammation in dogs with babesiosis [Pubmed: 23875771]
Arch Dis Child PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns [Bmj:Source]
Arch Dis Child? PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome [Bmj:Source]
Arch Dis Child PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography [Bmj:Source]
J Thorac Dis.? Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension [Pubmed:Pmc4015009]
Journal of Immunotoxicology Acacia ferruginea inhibits cyclophosphamide-induced immunosuppression and urotoxicity by modulating cytokines in mice [Pubmed:24873678]
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
Int J Neurosci. The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247]
Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits [Pubmed:25172523]
Nutr Res. Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits [Pubmed:25282128]
Experimental &amp; Molecular Medicine Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349]
Neural Regen Res.? Inflammatory response and neuronal necrosis in rats with cerebral ischemia [Pubmed:25422636]
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
CNS Neuroscience &amp; Therapeutics Peroxisome Proliferator-activated Receptor-γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive Rats [Pubmed:25611692]
Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats [Sciencedirect:S2090506814000268]
Canadian Journal of Physiology and Pharmacology Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism [Doi: Abs]
J Cell Biochem Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms [PubMed: 26177712]
Neurotoxicology Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats [PubMed: 25845300]
Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in [Science: Article]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140]
Experimental Eye Research The effect of triamcinolone acetonide or bevacizumab on the levels of proinflammatory cytokines after retinal laser photocoaCavia (Guinea pig )lation in pigmented rabbits [Pubmed:27296072]
Chinese Chemical Letters Screening the anti-gout traditional herbs from TCM using an in vitro method [science:S1001841716301450]
Am J Physiol Lung Cell Mol Physiol Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis [Pubmed:26993522]
Mol Neurobiol A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655]
Comparative Clinical Pathology Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets [article:10.1007]
Journal of?Cellular?Biochemistry Tumor Necrosis Factor‐Alpha Up‐Regulates ICAM‐1 Expression and Release in Intestinal Myofibroblasts by Redox‐Dependent and‐Independent Mechanisms [pubmed:26177712]
BMC Vet Res. Blood markers of fibrinolysis and endothelial activation in canine babesiosis. [pubmed:28363279]
Biomedicine & pharmacotherapy= Biomedecine & pharmacotherapie Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion [pubmed:28273631]
Pakistan journal of pharmaceutical sciences Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. [pubmed:29084680]
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422]
Molecular?Medicine?Reports Age?related differences in serum MFG?E8, TGF?β1 and correlation to the severity of atherosclerosis determined by ultrasound. [pubmed:29152651]
Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
International Journal of?Clinical?and Experimental Pathology Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study [ISSN:1936-2625/IJCEP0055881]
Oncotarget Global gene expression profile of cerebral ischemia-reperfusion injury in rat MCAO model [pubmed:29088811]
Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275]
Journal of ophthalmology Panretinal Photocoagulation Using Short-Pulse Laser Induces Less Inflammation and Macular Thickening in Patients with Diabetic Retinopathy [pubmed:28761761]
The Journal of Biological Chemistry Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]
Life?Sciences Lipoxin A4 may attenuate the progression of obesity-related glomerulopathy by inhibiting NF-κB and ERK/p38 MAPK-dependent inflammation [Pubmed:29499280]
International Journal of Molecular Medicine Aspirin modulates the inflammatory response in a thrombus?stimulated LMVEC model [Pubmed:29568915]
Clinical?And Experimental Pharmacology And Physiology? Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336]
Medicine (Baltimore) Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity [Pubmed:29953010]
留言咨询
幸运飞艇投注 平度市| 棋牌| 罗平县| 襄垣县| 江陵县| 南开区| 淄博市| 永安市| 晋宁县| 山丹县| 高陵县| 迭部县| 苗栗市| 尼木县| 株洲市| 红河县| 甘洛县| 天津工程信息网| 平阴县| 郸城县| 屏南县| 获嘉县| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>|