晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Advanced Glycation End Product (AGE)
AGEs; Advanced Glycation End Products
- 编号KSB353Ge11
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 试剂内容 包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma and other biological fluids
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围98.77-8,000ng/m
- 可适灵敏度38.2ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 7679 ¥ 12798 ¥ 23036 ¥ 44793 ¥ 76788
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
试剂盒中的抗体对晚期糖基化终末产物(AGE)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。
储存
抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。
配套试剂
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
NPB353Ge01 | 晚期糖基化终末产物(AGE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB353Ge01 | 晚期糖基化终末产物(AGE)活性蛋白 | Cell?culture;?Activity?Assays. |
PAB353Ge01 | 晚期糖基化终末产物(AGE)多克隆抗体 | WB; IHC; ICC; IP. |
LAB353Ge71 | 晚期糖基化终末产物(AGE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
LAB353Ge81 | 晚期糖基化终末产物(AGE)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
MAB353Ge21 | 晚期糖基化终末产物(AGE)单克隆抗体 | WB; IHC; ICC; IP. |
HEB353Ge | 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEB353Ge | 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
KSB353Ge11 | 晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Human Immunology | Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM) [PubMed: S0198885912005642] |
Experimental Dermatology | A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process [PubMed: 22017743] |
J Hum Nutr Diet. | Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. [Pubmed: 23829785] |
Gene | Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients. [Pubmed: 23721855] |
Gynecol Endocrinol. | Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392] |
Microvasc Res. | Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications [Pubmed:24984291] |
Free Radic Res. | Nitroxides prevent protein glycoxidation in vitro. [Pubmed:25363554] |
Sleep and Breathing | Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea [Pubmed:25566941] |
Chem Biol Interact | Ascorbic acid and protein glycation [PubMed: 26163454] |
Osteoarthritis Cartilage | Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone [PubMed: 26321377] |
journal of human hypertension | Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea [PubMed: 25994997] |
Redox Biol. | Glycation of bovine serum albumin by ascorbate [PubMed: 26202868] |
J Oral Pathol Med | Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886] |
J Agric Food Chem | Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats. [PubMed: 26207852] |
Kineziologija | ADVANCED GLYCATION END PRODUCTS'RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES [140251: ] |
Food Funct. | Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized rats [PubMed: 25920003] |
Molecular and Cellular Endocrinology | Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells [Pubmed:26952874] |
Advances in Experimental Medicine and Biology | Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury [Pubmed:27334732] |
Eur J Vasc Endovasc Surg | The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass Vein [Pubmed:26905625] |
molecular and cellular endocrinology | Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy. [pubmed:28087385] |
cellular physiology and biochemistry | Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine. [pubmed:28214842] |
General?Physiology?and?Biophysics | Glutathione?is the?main?endogenous?inhibitor?of?protein?glycation. [pubmed:28218612] |
Osteoarthritis?and?Cartilage | Establishment?of a?rabbit?model?to?study?the?influence?of?advanced?glycation?end?productsaccumulation?on?osteoarthritis?and the?protective?effect?of pioglitazone. [pubmed:26321377] |
Scientific Reports | Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway [pubmed:29097668] |
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment [pubmed:29138799] |
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