脂蛋白a(Lpa)活性蛋白

Active Lipoprotein, a (Lpa)

Lp(a); AK38; APOA; LP; Sinking Pre-β-Lipoprotein

  • 脂蛋白a(Lpa)活性蛋白 产品包装(模拟)
  • 脂蛋白a(Lpa)活性蛋白 产品包装(模拟)
  • APA842Hu01.png Figure. SDS-PAGE
  • 脂蛋白a(Lpa)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Lipoprotein, a (Lpa) is a lipoprotein subclass. Lpa is assembled at the hepatocyte cell membrane surface, while other scenarios exist with regard to the location of assembly. It mainly exists in plasma. Lpa contributes to the process of atherogenesis. It also may enhance coagulation by inhibiting the function of tissue factor pathway inhibitor. Lpa carries cholesterol and binds atherogenic proinflammatory oxidized phospholipids as a preferential carrier of oxidized phospholipids in human plasma, which attract inflammatory cells to vessel walls and leads to smooth muscle cell proliferation. Moreover, Lpa also is hypothesized to be involved in wound healing and tissue repair, interacting with components of the vascular wall and extra cellular matrix. Besides, Fibronectin (FN) has been identified as an interactor of Lpa, thus a binding ELISA assay was conducted to detect the interaction of recombinant human Lpa and recombinant human FN. Briefly, Lpa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to FN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Lpa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of Lpa and FN was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of Lpa with FN.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA842Hu01 脂蛋白a(Lpa)活性蛋白 Cell?culture;?Activity?Assays.
RPA842Hu01 脂蛋白a(Lpa)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA842Hu01 脂蛋白a(Lpa)多克隆抗体 WB; IHC; ICC; IP.
MAA842Hu22 脂蛋白a(Lpa)单克隆抗体 WB; IHC; ICC; IP.
SEA842Hu 脂蛋白a(Lpa)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Molecular Sciences Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R [Pubmed:27187377]
Evidence-Based Complementary and Alternative Medicine Danshen?(Salvia?miltiorrhiza)?Compounds?Improve?the?Biochemical?Indices?of the?Patients?with?Coronary?HeartDisease. [Pubmed:27366196]
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