Active Leptin (LEP)

OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

  • 瘦素(LEP)活性蛋白 产品包装(模拟)
  • 瘦素(LEP)活性蛋白 产品包装(模拟)
  • APA084Hu01.jpg SDS-PAGE图
  • 瘦素(LEP)活性蛋白 Figure. Western Blot; Sample: Recombinant LEP, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证


Leptin is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Many of leptin's effects are mediated through neuropeptide- containing neurons and neuropeptide receptors in the hypothalamus. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Besides, Hexokinase 3, White Cell (HK3) has been identified as an interactor of LEP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human LEP and recombinant human HK3. Briefly, LEP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HK3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LEPpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of LEP and HK3 was shown in Figure 1, and this effect was in a dose dependent manner.


Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.






编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA084Hu01 瘦素(LEP)活性蛋白 Cell?culture;?Activity?Assays.
RPA084Hu01 瘦素(LEP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA084Hu01 瘦素(LEP)多克隆抗体 WB; IHC; ICC; IP.
LAA084Hu71 瘦素(LEP)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA084Hu81 瘦素(LEP)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA084Hu22 瘦素(LEP)单克隆抗体 WB; IHC; ICC; IP.
MAA084Hu21 瘦素(LEP)单克隆抗体 WB; IHC; ICC; IP.
SEA084Hu 瘦素(LEP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA084Hu 瘦素(LEP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA084Hu01 瘦素(LEP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".


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