胱天蛋白酶3(CASP3)活性蛋白

Active Caspase 3 (CASP3)

CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1

  • 胱天蛋白酶3(CASP3)活性蛋白 产品包装(模拟)
  • 胱天蛋白酶3(CASP3)活性蛋白 产品包装(模拟)
  • APA626Hu01.png Figure. SDS-PAGE
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活性实验

Caspase 3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. Caspase 3 can hydrolyze the peptide substrate acetyl-Asp-Glu-Val-Asp-p-nitroanilide (Ac-DEVD-pNA) resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline has a high absorbance at 405nm. Thus the activity of recombinant human caspase 3 can be measured by calculate the concentration of the pNA released from the substrate. The reaction was performed in adding 50μL 2×buffer (50mM HEPES, 100mM NaCl, 10mM DTT, 2mM EDTA, 10% glycerol) to 96 well plates, then add 50μL various concentration of caspe 3 (diluted by 1×buffer, 25mM HEPES, 50mM NaCl, 5mM DTT, 1mM EDTA, 5% glycerol) to each well, finally, add 5μL 4mmol Ac-DEVD-pNA to each well. Cover the 96 well plates and incubate at 37℃ for 2h. p-Nitroaniline (pNA) standard curve prepare by double dilute 200μM pNA with 1×buffer and record the OD value at 405nm. Calculate the caspase 3 activity in pmol of pNA released per min per μg recombinat human caspase 3.
The specific activity of recombinant human caspase 3 is 2196pmol/min/μg.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA626Hu01 胱天蛋白酶3(CASP3)活性蛋白 Cell?culture;?Activity?Assays.
RPA626Hu01 胱天蛋白酶3(CASP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA626Hu02 胱天蛋白酶3(CASP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA626Hu03 胱天蛋白酶3(CASP3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA626Hu02 胱天蛋白酶3(CASP3)多克隆抗体 WB; IHC; ICC; IP.
PAA626Hu01 胱天蛋白酶3(CASP3)多克隆抗体 WB; IHC; ICC; IP.
LAA626Hu81 胱天蛋白酶3(CASP3)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA626Hu71 胱天蛋白酶3(CASP3)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA626Hu21 胱天蛋白酶3(CASP3)单克隆抗体 WB; IHC; ICC; IP.
MAA626Hu22 胱天蛋白酶3(CASP3)单克隆抗体 WB; IHC; ICC; IP.
SEA626Hu 胱天蛋白酶3(CASP3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA626Hu01 胱天蛋白酶3(CASP3)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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