脂联素受体1(ADIPOR1)活性蛋白

Active Adiponectin Receptor 1 (ADIPOR1)

ACDCR1; CGI45; PAQR1; TESBP1A; TESBP1-A; Progestin and AdipoQ Receptor Family Member I

  • 脂联素受体1(ADIPOR1)活性蛋白 产品包装(模拟)
  • 脂联素受体1(ADIPOR1)活性蛋白 产品包装(模拟)
  • APA147Hu02.jpg SDS-PAGE图
  • 脂联素受体1(ADIPOR1)活性蛋白 Figure. Western Blot; Sample: Recombinant ADIPOR1, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

ADIPOR1 (Adiponectin receptor protein 1) is a receptor for ADP (30kDa adipocyte complement-related protein), an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Besides, human ADP and rat ADP exist similarities in amino acid sequence with the identity of 83.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ADIPOR1 and recombinant rat ADP. Briefly, ADIPOR1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ADP-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ADIPOR1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ADIPOR1 and ADP was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA147Hu01 脂联素受体1(ADIPOR1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA147Hu02 脂联素受体1(ADIPOR1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA147Hu02 脂联素受体1(ADIPOR1)活性蛋白 Cell?culture;?Activity?Assays.
PAA147Hu01 脂联素受体1(ADIPOR1)多克隆抗体 WB; IHC; ICC; IP.
PAA147Hu02 脂联素受体1(ADIPOR1)多克隆抗体 WB; IHC; ICC; IP.
LAA147Hu71 脂联素受体1(ADIPOR1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA147Hu22 脂联素受体1(ADIPOR1)单克隆抗体 WB; IHC; ICC; IP.
SEA147Hu 脂联素受体1(ADIPOR1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
PLoS One Topiramate-Induced Modulation of Hepatic Molecular Mechanisms: An Aspect for Its Anti-Insulin Resistant Effect [PubMed: 22649556]
Applied Physiology, Nutrition, and Metabolism Estimation?of?ellagic acid?and/or?repaglinide?effects?on?insulin?signaling,?oxidative stress, andinflammatory?mediators?of?liver,?pancreas,?adipose tissue, and?brain?in?insulin?resistant/type 2 diabetic rats. [pubmed:28092161]
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