70kDa热休克蛋白1A(HSPA1A)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A)

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

  • 70kDa热休克蛋白1A(HSPA1A)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 70kDa热休克蛋白1A(HSPA1A)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对70kDa热休克蛋白1A(HSPA1A)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
RPB081Po01 70kDa热休克蛋白1A(HSPA1A)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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MAB081Po21 70kDa热休克蛋白1A(HSPA1A)单克隆抗体 WB; IHC; ICC; IP.
SEB081Po 70kDa热休克蛋白1A(HSPA1A)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB081Po01 70kDa热休克蛋白1A(HSPA1A)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journal of Neuroinflammation Identification of potential biomarkers of gold nanoparticle toxicity in rat brains [PubMed: 22691312]
Mediators Inflamm.? Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs [Pubmed:Pmc4324980]
Eur J Pharmacol. Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats [Pubmed:24508522]
Pharmacol Rep. Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice [Pubmed:25443743]
Advances in Medical Sciences A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267]
Archives of Medical Research Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533]
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